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Registros recuperados: 176 | |
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Chiaratti,Marcos R.; Macabelli,Carolina H.; Augusto Neto,José Djaci; Grejo,Mateus Priolo; Pandey,Anand Kumar; Perecin,Felipe; Collado,Maite del. |
Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oocyte; Germline; Mitochondrial dynamics; MtDNA; Metabolism. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200308 |
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Gavira,Rodrigo S. B.; Andrade,Denis V.. |
We examined the effects of meal size on the postprandial metabolic response of the lancehead Bothrops alternatus (Duméril, Bibron & Duméril, 1894), fed mice equaling to 5, 10, 20, and 40% of the snake's body mass. The maximum O2 consumption rates measured during digestion increased with meal size, reaching levels up to 2.8-7.8-fold higher than the metabolic rate measured during fasting. Specific Dynamic Action (SDA) duration also increased with meal size, lasting from 54 to 212 hours to complete. Under our experimental conditions, 30ºC, the majority of our snakes failed to completely digest prey with a relative size of 40%. The SDA coefficient ranged from 17 to 27% of the energy content of the meal and was not affected by meal size. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolism; Postprandial; Prey size; Snakes. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702013000300005 |
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Bazotte,R.B.; Batista,M.R.; Curi,R.. |
Naomi Shinomiya Hell was the first researcher to investigate the physiological adaptations to a meal-feeding scheme (MFS) in Brazil. Over a period of 20 years, from 1979 to 1999, Naomi's group determined the physiological and metabolic adaptations induced by this feeding scheme in rats. The group showed the persistence of such adaptations even when MFS is associated with moderate exercise training and the performance to a session of intense physical effort. The metabolic changes induced by the feeding training were discriminated from those caused by the effective fasting period. Naomi made an important contribution to the understanding of the MFS but a lot still has to be done. One crucial question still remains to be satisfactorily answered: what is the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meal-feeding scheme; Metabolism; Exercise; Fasting; Liver. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900001 |
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Chippari-Gomes,Adriana R.; Leitão,Marco Aurélio B.; Paula-Silva,Maria de Nazaré; Mesquita-Saad,Lenise S. B. de; Almeida-Val,Vera Maria F.. |
In this paper, we describe the enzyme levels and isozyme distribution in skeletal and heart muscle of Satanoperca aff. jurupari, (Cichlidae, subgroup Geophaginae). LDH and CS were measured in skeletal and heart muscle. Starch gel electrophoresis was used to determine the isozyme/allozyme patterns in different tissues; LDH, MDHs, PGM, PGI, ADH, G-6-PDH and SOD were screened for the numbers of loci, presence of alleles, and tissue specificity. The LDH/CS ratio in heart and skeletal muscle were 173.36 and 6.1, respectively, indicating anaerobic metabolism in the former and aerobic metabolism in the latter muscle. No inhibition by pyruvate (based on the ratios of LDH activity with 1 mM and 10 mM pyruvate) was detected in heart and skeletal muscle, indicating... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cichlid; Plasticity; Metabolism; Enzymes; Amazon. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100005 |
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Lopes,Edna; Sousa,Raimundo Vicente de; Zangeronimo,Márcio Gilberto; Pereira,Andressa Naira de Jesus; Coelho,Mariana de Resende; Ferreira,Matheus Soares da Silva; Lima,Renato Ribeiro; Marcondes,Fernanda Klein; Napimoga,Marcelo Henrique; Pereira,Luciano José. |
This study aimed at evaluating the effect of ractopamine (RAC) on metabolism, zootechnical performance, body composition, and behavior in Wistar rats submitted to acute and chronic restrain stress. The oral dose of 5 mg/kg of RAC was administered in periods of 0, 7, 14, 21, and 28 days. The elevated plus-maze test (EPMT) was used for behavioral assessment. Blood, carcass and viscera characteristics were evaluated. Insulin-dependent glucose transporters (GLUT-4) were semi-quantified by Western Blot in epididymal adipocytes. RAC periods associated with chronic stress increased the GLUT-4 protein expression in adipose tissue in a time-dependent manner (P=0.01), i.e., the longer the RAC addition period, the higher the GLUT-4 concentration in chronically... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Β-adrenergic agonist; Ractopamine; Zootechnical performance; Metabolism; Elevated Plus-Maze Tests. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300406 |
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Drumond,Mariana Martins; Naves,Luciana de Paula; Ribeiro,Paula Adriane Perez; Oliveira,Marinez Moraes de; Okamura,Daniel; Resende,Anaise Emanuele; Cantarelli,Vinicius de Souza; Rosa,Priscila Vieira. |
ABSTRACT An experiment was conducted during 60 days with forty pacu males fed diets supplemented with increasing concentrations of ractopamine (0.00, 11.25, 22.50, 33.75, and 45.00 mg kg−1). Eight fish were evaluated for each experimental diet. Performance and survival rate of the fish were measured. At the end of the experiment, blood was collected to determine the levels of cortisol, triacylglycerol, and protein. Moreover, the liver was collected to determine the activities of glucose-6-phosphate dehydrogenase and malic enzymes. The fillets were collected to determine chemical composition, and histologic cuts were analyzed to verify muscle growth and deposition of adipose tissue between muscle fibers. Increasing concentrations of dietary ractopamine did... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Beta-adrenergic agonist; Enzyme; Metabolism; Stress. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100100 |
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Soares,P.C.; Gouveia,L.N.F.; Silva,T.G.P.; O. Filho,E.F.; Carvalho,F.F.R.; Batista,Â.M.V.; Lima Júnior,D.M.; Cardoso,D.B.. |
ABSTRACT The objective of this study was to evaluate the metabolic response of native lambs fed Tifton 85 hay (Cynodon dactylon) or Maniçoba hay (Manihot pseudoglaziovii) associated with spineless cactus (Nopalea cochenillifera Salm Dyck). Sixteen Morada Nova lambs were used, with a mean initial weight of 19.36±1.48kg, distributed in a randomized block design, with two treatments and eight replications. Dry matter intake was higher for lambs fed Maniçoba hay compared to Tifton hay, while the body weight at slaughter was not influenced by diets. Regarding the blood metabolites, only the serum concentration of urea was influenced, with higher value observed in the lambs that fed the diet containing Tifton hay. When analyzing blood biomarkers, in the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blood biomarkers; Clinical biochemistry; Metabolism; Native breeds; Nutrition; Small ruminants. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100215 |
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Astessiano,Ana Laura; Pérez-Clariget,Raquel; Espasandín,Ana Carolina; López-Mazz,Carlos; Soca,Pablo; Carriquiry,Mariana. |
The objective of this study was to evaluate the effect of a short-term supplementation with rice bran (2 kg/cows/day) on the endocrine and metabolic profiles and hepatic gene expression, associated with the reproductive response in beef cows in grazing conditions. Thirty-eight primiparous beef cows (Hereford, Angus and Hereford × Angus) were used in a randomized block design according to genotype, calving date and body condition score at calving (BCS). At 64±14 days postpartum (day 0 of the experiment), cows were assigned to two nutritional treatments: i) control, grazing native pastures (CON, n=19) and ii) supplemented (SUP, n = 19) for 21 days (days 1 to 21 of the experiment). Blood samples were collected at 0, 7 and 22 days and liver biopsies were... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: GH-IGFI axis; Metabolism; MRNA. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000400003 |
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Cadiz, Laura; Zambonino-infante, Jose-luis; Quazuguel, Patrick; Madec, Lauriane; Le Delliou, Herve; Mazurais, David. |
Several physiological functions in fish are shaped by environmental stimuli received during early life. In particular, early-life hypoxia has been reported to have long-lasting effects on fish metabolism, with potential consequences for fish life history traits. In the present study, we examine whether the synergistic stressors hypoxia (40% and 100% air saturation) and temperature (15° and 20 °C), encountered during early life, could condition later metabolic response in European sea bass (Dicentrarchus labrax) juveniles. Growth rate and metabolic parameters related to carbohydrate and lipid metabolism in the liver were investigated at the juvenile stage under normoxic and chronic hypoxic conditions. Juvenile growth rates were significantly lower... |
Tipo: Text |
Palavras-chave: Hypoxia; Developmental plasticity; Metabolism; European sea bass. |
Ano: 2018 |
URL: http://archimer.ifremer.fr/doc/00404/51536/52126.pdf |
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Prodocimo,Viviane; Souza,Carine F.; Pessini,Cristina; Fernandes,Luiz Claudio; Freire,Carolina A.. |
The marine-estuarine species of pufferfishes Sphoeroides testudineus and S. greeleyi are very efficient osmoregulators. However, they differ with respect to their tolerance of salinity reduction. During low tide S. testudineus remains in diluted estuarine waters, whereas S. greeleyi returns to seawater (SW). The hypothesis tested here was that the short-term mobilization of metabolic substrates stored in their main osmoregulatory organs would correlate with this differential tolerance. Fishes exposed to 5 (for 6 h) were compared to those kept in 35. Branchial and renal contents of triglycerides, protein and glycogen were evaluated, and total ATPase activity accounted for the tissues' metabolism. Plasma osmolality, chloride and glucose, hematocrit, and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish gill; Fish kidney; Glycogen; Metabolism; Osmoregulation; Tetraodontidae. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252008000400009 |
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Nascimento,Cláudia M. Oller do; Ribeiro,Eliane B.; Oyama,Lila M.. |
Approximately 40% of the total energy consumed by western populations is represented by lipids, most of them being ingested as triacylglycerols and phospholipids. The focus of this review is to analyze the effect of the type of dietary fat on white adipose tissue metabolism and secretory function, particularly on haptoglobin, TNF-α, plasminogen activator inhibitor-1 and adiponectin secretion. Previous studies have demonstrated that the duration of the exposure to the high-fat feeding, amount of fatty acid present in the diet and the type of fatty acid may or may not have a significant effect on adipose tissue metabolism. However, the long-term or short-term high fat diets, especially rich in saturated fatty acids, probably by activation of toll-like... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adipokines; High fat diets; Metabolism; White adipose tissue. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300010 |
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Morita, R; Jones, R. |
The nitrifying bacteria were found to survive 24 weeks in the absence of ammonium without decreasing their number of cell size. Because H sub(2), CO, and CH sub(4) are present in the marine environment, these substrates were investigated as a possible source of the energy of maintenance for the nitrifying bacteria. super(14)CO and super(14)CH sub(4) were found to be oxidized by the nitrifiers. N-serve was found to inhibit the oxidation of CO. Using the nitrifiers' ability to oxide CO, a method for the determination of the nitrification rate was developed. The ability of nitrifiers to oxidize CO may play a significant role in the cycling of CO sub(2) in the marine environment. Whether CO and CH sub(4) oxidation play a role in the survival of nitrifiers in... |
Tipo: Text |
Palavras-chave: Methane; Carbon dioxide; Survival; Bacteria; Nitrification; Nitrogen cycle; Oxidation; Carbon cycle; Biogeochemistry; Metabolism. |
Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-975.pdf |
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We have studied the metabolism of diglycine and triglycine in the isolated non-filtering rat kidney. Kidneys from adult male Wistar Kyoto rats weighing 250-350 g were perfused with Krebs-Henseleit solution containing either 1 mM diglycine or triglycine. The analysis of the peptide residues and their components was performed using an amino acid microanalyzer utilizing ion exchange chromatography. Diglycine was degraded to a final concentration of 0.09 mM after 120 min (91%); this degradation occurred predominantly during the first hour, with a 56% reduction of the initial concentration. The metabolism of triglycine occurred similarly, with a final concentration of 0.18 mM (82%); during the first hour there was a 67% reduction of the initial concentration of... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Non-filtering kidney; Dipeptide; Tripeptide; Metabolism; Peptidases. |
Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000200005 |
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Fuhrmann, Marine; Delisle, Lizenn; Petton, Bruno; Corporeau, Charlotte; Pernet, Fabrice. |
The Pacific oyster, Crassostrea gigas, is an osmoconforming bivalve exposed to wide salinity fluctuations. The physiological mechanisms used by oysters to cope with salinity stress are energy demanding and may impair other processes, such as defense against pathogens. This oyster species has been experiencing recurrent mortality events caused by the Ostreid herpesvirus 1 (OsHV-1). The objectives of this study were to investigate the effect of salinity (10, 15, 25 and 35‰) on energetic reserves, key enzyme activities and membrane fatty acids, and to identify the metabolic risk factors related to OsHV-1-induced mortality of oysters. Acclimation to low salinity led to increased water content, protein level, and energetic reserves (carbohydrates and... |
Tipo: Text |
Palavras-chave: Bivalve; Disease; Environment; Metabolism; Mortality risk; Salinity. |
Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00427/53886/54958.pdf |
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White, D. |
Microbes in nature are often concentrated on surfaces where they exist in microcolonies of mixed composition. The assay of these mixed microbial consortia by utilizing biochemical components eliminates the problems associated with cultural selectivity of viable counts or the requirement for quantitative release from surfaces required in some microscopic techniques. The assay of cellular components restricted to subsets of the microbial community gives insights into the community structure of the biofilm whereas assay of cellular components common to all cells can be used for biomass estimations. Formation of endogenous storage materials can be utilized as measures of nutritional status and rates of formation or turnover of specific "signatures" from... |
Tipo: Text |
Palavras-chave: Bacteria; Fouling; Bioassays; Metabolism; Surfaces; Bacteria collecting devices; Culture media; Microbiological analysis. |
Ano: 1984 |
URL: http://archimer.ifremer.fr/doc/1984/acte-963.pdf |
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Jebbar, Mohamed; Hickman-lewis, Keyron; Cavalazzi, Barbara; Taubner, Ruth-sophie; Rittmann, Simon K.-m. R.; Antunes, Andre. |
The icy moons of the outer Solar System harbor potentially habitable environments for life, however, compared to the terrestrial biosphere, these environments are characterized by extremes in temperature, pressure, pH, and other physico-chemical conditions. Therefore, the search for life on these icy worlds is anchored on the study of terrestrial extreme environments (termed “analogue sites”), which harbor microorganisms at the frontiers of polyextremophily. These so-called extremophiles have been found in areas previously considered sterile: hot springs, hydrothermal vents, acidic or alkaline lakes, hypersaline environments, deep sea sediments, glaciers, and arid areas, amongst others. Such model systems and communities in extreme terrestrial environments... |
Tipo: Text |
Palavras-chave: Extremophiles; Prokaryotes; Metabolism; Diversity; Adaptation; Space explorat. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71771/70275.pdf |
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Registros recuperados: 176 | |
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